Rat TrxR1 (Thioredoxin Reductase 1) CLIA Kit (E-CL-R0634)

Name: Rat TrxR1 (Thioredoxin Reductase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0634
Price: 180.0 USD
Availability: InStock

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Rat TrxR1 (Thioredoxin Reductase 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat TrxR1 in samples.No significant cross-reactivity or interference between Rat TrxR1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54859.000	62941.000	58900.000	58876
1000	25015.000	26475.000	25745.000	25721
500	11900.000	10212.000	11056.000	11032
250	5781.000	5837.000	5809.000	5785
125	2974.000	2754.000	2864.000	2840
62.5	1427.000	1229.000	1328.000	1304
31.25	682.000	700.000	691.000	667
0	24.000	24.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.870	312.860	818.220	103.910	340.670	786.920
Standard deviation	9.160	24.000	87.710	11.800	36.960	73.580
CV（%）	9.660	7.670	10.720	11.360	10.850	9.350

Recovery

The recovery of Rat TrxR1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-108	98
EDTA plasma （n=8）	88-104	95
Cell culture media （n=8）	98-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat TrxR1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-102	90-104	87-98
1:2	Average	97	96	93
1:4	Range	96-109	92-104	95-107
1:4	Average	101	97	101
1:8	Range	97-113	102-117	85-100
1:8	Average	103	109	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TrxR1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat TrxR1. You can calculate the concentration of Rat TrxR1 in the samples by comparing the RLU value of the samples to the standard curve.

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