Rat TSH (Thyroid Stimulating Hormone) CLIA Kit (E-CL-R0647)

Name: Rat TSH (Thyroid Stimulating Hormone) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0647
Price: 180.0 USD
Availability: InStock

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Rat TSH (Thyroid Stimulating Hormone) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat TSH in samples.No significant cross-reactivity or interference between Rat TSH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	37111.000	37169.000	37140.000	37112
10	11292.000	13650.000	12471.000	12443
5	5738.000	5398.000	5568.000	5540
2.5	2430.000	2744.000	2587.000	2559
1.25	1562.000	1344.000	1453.000	1425
0.63	934.000	892.000	913.000	885
0.31	616.000	680.000	648.000	620
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.060	3.050	8.070	1.010	2.910	7.800
Standard deviation	0.130	0.360	0.860	0.100	0.330	0.720
CV（%）	12.260	11.800	10.660	9.900	11.340	9.230

Recovery

The recovery of Rat TSH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-111	101
EDTA plasma （n=8）	91-105	99
Cell culture media （n=8）	97-113	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat TSH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-107	90-103	93-105
1:2	Average	101	97	98
1:4	Range	99-117	97-112	99-111
1:4	Average	107	105	106
1:8	Range	94-105	97-111	99-113
1:8	Average	100	103	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the RLU value of the samples to the standard curve.

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