Current Position:Home>>Kits>>ELISA Kits>> Rat VWF(Von Willebrand Factor) ELISA Kit

Rat VWF(Von Willebrand Factor) ELISA Kit

Cat.No.:E-EL-R1079
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R1079

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat VWF in samples. No significant cross-reactivity or interference between Rat VWF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat VWF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VWF. You can calculate the concentration of Rat VWF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VWF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VWF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.47	1.56	3.62	0.43	1.57	3.87
Standard deviation	0.03	0.07	0.12	0.03	0.09	0.19
CV (%)	6.38	4.49	3.31	6.98	5.73	4.91

Recovery

The recovery of Rat VWF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-101	94
EDTA plasma (n=8)	85-97	90
Cell culture media (n=8)	89-102	94

Linearity

Samples were spiked with high concentrations of Rat VWF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	F8VWF, VWD
Research Area	Cancer, Cardiovascular, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ACTA PHARMACOLOGICA SINICA (2020) IF: 5.064
Salvianolic acid A prevented cerebrovascular endothelial injury caused by acute ischemic stroke through inhibiting the Src signaling pathway

DOI: 10.1038/s41401-020-00568-2

PMID: 33303991
Sample: Serum
Cells (2019) IF: 5.656
The Role of GLP1 in Rat Steatotic and Non-Steatotic Liver Transplantation from Cardiocirculatory Death Donors

DOI: 10.3390/cells8121599

Sample: plasma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
Extracellular Vesicles as an Index for Endothelial Injury and Cardiac Dysfunction in a Rodent Model of GDM

DOI: 10.3390/ijms23094970

PMID: 35563365
Sample: plasma
Scientific Reports (2021) IF: 4.38
Validation of the relationship between coagulopathy and localization of hydroxyethyl starch on the vascular endothelium in a rat hemodilution model

DOI: 10.1038/s41598-021-89889-8

PMID: 34021192
Sample: Plasma
Drug Design Development and Therapy (2015) IF: 3.028
Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats

DOI: 10.2147/DDDT.S87772

Sample: Serum
Food & Nutrition Research (2015) IF: 2.162
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats

DOI: 10.3402/fnr.v59.29046

Sample: Serum
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020) IF: 2.375
Chronic intermittent hypoxia vs chronic continuous hypoxia: Effects on vascular endothelial function and myocardial contractility

DOI: 10.3233/CH-190706

Sample: serum
MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402
Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors

DOI: 10.1007/s11033-020-05746-3

PMID: 32892299
Sample: Serum
Molecular Medicine Reports (2017) IF: 1.692
Oleanolic acid alleviates diabetic rat carotid artery injury through the inhibition of NLRP3 inflammasome signaling pathways

DOI: 10.3892/mmr.2017.7594

PMID: 28944913
Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

R************iSubmitted [ Mar 14 2019 ]

Asked: I have a question about detectable antigen of this kit. Can this kit detect only VWF multimer ? or all kinds of molecular weight of VWF including dimer or monomer ? I\'m very happy if you have information.

adminSubmitted [ Mar 14 2019 ]

Answered: This is Yumi from Elabscience~thanks for your inquiry,this kit can recognize total VWF, including dimer or monomer forms.If any question,please send email to yumi@elabscience.com

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha