SEM(Nitrofurazone Metabolite) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E005

      Size:
      • 96T
      Qty:
      - +
      Price: $526

      Sensitivity: 0.05 ppb

      Sample type: Tissue, Liver, Milk(liquid/powder), Egg powder, Feed, Honey, Casing

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Indirect-Competitive-ELISA as the method. It can detect SEM in samples, such as honey, tissue, milk, fodder, etc. This kit is composed of MicroELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with SEM. During the reaction, SEM in the samples or standard competes with SEM on the solid phase supporter for sites of SEM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of SEM. The concentration of SEM in the samples can be calculated by comparing the OD of the samples to the standard curve.

       

      Technical indicator

      Sensitivity: 0.05 ppb (ng/mL)

      Reaction mode: 25, 45 min~ 15 min

      Detection limit: Tissue, liver---0.1 ppb, Honey/milk/casing---0.1 ppb,

                                   Milk powder/egg powder/feed--0.1 ppb, Fish and shrimp---0.15 ppb

      Cross-reactivity: Nitrofurazone metabolite---100%, Furazolidone metabolite---0.1%,

                                      Nitrofurantoin metabolite---0.1%, Furaltadone metabolite---0.1%

      Sample recovery rate: Tissue/liver---90%±15%,Honey/milk/casing---80%±15%; 

                                                Milk powder/egg powder/feedr--85%±25%


      Kits components

       

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0ppb,0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb)

      Derivatization Reagent

      10 mL

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators/Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Ethyl acetate, N-hexane, Methanol,  NaOH,Concentrated HCl, K2HPO4•3H2O, K2Fe (CN) 5(NO) ▪2H2O, ZnSO4•7H2O.



      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration  date is on the packing box.

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