SM (Streptomycin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E031

      Size:
      • 96T
      Qty:
      - +
      Price: $360

      Sensitivity: 0.10 ppb

      Sample type: Tissue, Honey, Milk,Milk powder

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Streptomycin (SM) in tissues, honey and other samples. This kit is composed of ELISA Microplate pre-coated with coupled antigen, HRP conjugate, antibody, standard and other supplementary reagents. During the detection, after adding standard or sample solution, SM in the samples competes with pre-coated coupled antigen on the Micro ELISA Plate for SM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of SM. The residual quantity of SM in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.1 ppb (ng/mL)

      Reaction mode: 25, 30 min~30 min~ 15 min

      Detection limit: Tissue ---4 ppb, Honey---2 ppb, Milk, Milk powder ---5 ppb.

      Cross-reactivity: Streptomycin---100%, Dihydrostreptomycin---100%, Kalamycin---6.3%,Gentamicin ---2.5%.

      Sample recovery rate: Tissue, Honey, Milk---85%±15%.


      Kits components

      Item

      Specifications

      ELISA Microplate

      96 wells

      Standard Liquid 

       1 mL each

      (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)

      High Concentrated Standard (1 ppm)

      1 mL

      HRP Conjugate

      11 mL

      Antibody Working Solution 

      5.5 mL

      Substrate Reagent A 

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution 

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      5×Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      Micropipettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL).

      Reagents: NaOH, Na2HPO412H2O, NaH2PO42H2O, N-hexane, Dichloromethane, Acetonitrile, Phosphoric acid.

       


      Storage and valid period

      Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration date is on the packing box.


                            


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