TMP (Trimethoprim) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E023

      Size:
      • 96T
      Qty:
      - +
      Price: $360

      sample_type: Feed,Tissue,Serum,Urine

      Sensitivity: 0.015 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Trimethoprim (TMP) in samples, such as tissue, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, TMP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TMP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TMP. The concentration of TMP in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.015 ppb

      Reaction mode: 37℃, 45 min~15 min

      Reaction rate: Trimethoprim (TMP) ---100%

      Detection limit:  Feed---0.8 ppb; Tissue---0.2 ppb; Serum, Urine---0.2 ppb

      Cross-reactivity: Trimethoprim ---100%, Sulfapyridine---<0.1%, Sulfadiazine---<0.1%, 

                                    Sulfisoxazole---<0.1%, Sulfathiazole ---<0.1%, Sulfamerazine---<0.1%,

                                   Sulfadoxine---<0.1%,

      Sample recovery rate: Feed---85%±10%; Serum, Urine---85%±10%; Tissue --- 85%±15%


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.015 ppb, 0.045 ppb, 0.135 ppb, 0.405 ppb, 1.215 ppb)

      HRP Conjugate

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      2×Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other materials required but not supplied

      Instruments: Microtiter plate reader, Printer, Homogenizer, Nitrogen Evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision pipettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL).

      Reagents: Anhydrous methanol, N-hexane, NaOH, concentrated HCl.



      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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