Total Antioxidant Capacity Colorimetric (T-AOC) Assay Kit (FRAP method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K225-M

      Size:
      • 48T
      • 96T
      Qty:
      - +
      Price: $120

      Detection method: Colorimetric method

      Detection instrument: Microplate reader

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      General information

      Detection significance

      There are two types of antioxidant systems in the body, one is the enzymatic antioxidant system, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px). The other is a non-enzymatic antioxidant system, including uric acid, vitamin C, vitamin E, glutathione, bilirubin, α-lipoic acid and carotenoids. Antioxidant capacity is thought to be the cumulative effect of all antioxidants in blood and body fluids

      Detection principle

      Fe3+-TPTZ can be reduced by antioxidants and produce blue Fe2+-TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm.

      Operation procedures

      Operation steps

      1. Preparation of standard

      Dilute 100 mmol/L FeSO4 solution with distilled water (See Note b) to a serial concentration. The recommended dilution gradient is as follows: 0, 0.3, 0.6, 0.9, 1.2, 1.8, 2.1, 2.5 mmol/L.

       

      2. Operation table

       

      Standard well

      Sample well

      Fe2SO4-7H2O Standard solution with different concentration (μL)

      5

       

      Sample (µL)

       

      5

      FRAP working solution (μL)

      180

      180

      Incubate at 37 ºC for 3~5 min, then measure the OD values of each well with Microplate reader at 593 nm (or choose an optimal wavelength between 590~600 nm).

      Note: a. A standard curve is required for every batch of experiments.

      b. For serum, plasma, saliva, urine and other liquid samples, it is recommended to use distilled water or 1×PBS to prepare the standard solution. For cell or tissue samples, it is recommended to use homogenate medium to prepare the standard solution.

      Performance characteristics

      Technical parameter

      Detection range 0.049-2.5 mmol/L Average inter-assay CV 8.1%
      Sensitivity 0.049 mmol/L Average intra-assay CV 3.9%
      Average recovery rate 103%
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