Total Bile Acid (TBA) Colorimetric Assay Kit
Price: $ 240
Price: $ 180
Size:
96T
48T
Quantity:
- Sample Type: serum;animal tissue
- Detection Instrument: Microplate reader (400-410 nm)
- Reasearch Areas: Liver Renal function biomarkers
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For research use only. Order now, ship in 3 days
Product Details
Detection Principle | With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid. | ||||||||||||||
Synonyms | TBA | ||||||||||||||
Sample Type | serum,animal tissue | ||||||||||||||
Sensitivity | 2.05 μmol/L | ||||||||||||||
Detection Range | 2.05-120 μmol/L | ||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||
Assay Time | 25 min | ||||||||||||||
Precision | inter-assay CV: 3.7% | intra-assay CV: 3.4% | ||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl) | ||||||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||||||
Valid Period | 12 months | ||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M,pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
Citations
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Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
IF:6.706
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Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota
IF:5.561
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PMID:36230124
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Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice
IF:5.318
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San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress
IF:2.65
Journal:Evidence-based Complementary and Alternative Medicine