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Total Bile Acid (TBA) Colorimetric Assay Kit

Cat:E-BC-K181-M Citations (4)
Manual MSDS

Price: $ 240

Price: $ 180

Size:
96T 48T
Quantity:
  • Sample Type: serum;animal tissue
  • Detection Instrument: Microplate reader (400-410 nm)
  • Reasearch Areas: Liver Renal function biomarkers
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Product Details
Detection Principle With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Synonyms TBA
Sample Type serum,animal tissue
Sensitivity 2.05 μmol/L
Detection Range 2.05-120 μmol/L
Detection Method Colorimetric method
Assay Time 25 min
Precision inter-assay CV: 3.7% | intra-assay CV: 3.4%
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Dog serum 1
Mouse serum 1
Rat serum 1
Bovine serum 1
10% mouse liver 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M,pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.