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Total Bilirubin (TBIL) Colorimetric Assay Kit

  • Cat.No.:E-BC-K760-M

  • Detection instrument: Microplate reader (565 nm)

To Purchase E-BC-K760-M

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  • 96T
  • 48T
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Detection principle

Bilirubin is one of the important components of bile. It is the degradation product of hemoglobin in various heme proteins under the action of a series of enzymes. It is important to the digestion and absorption of lipids and the formation of yellow distemper. Bilirubin comes in two forms: water-soluble and water-insoluble. Bilirubin has powerful antioxidant, anti-inflammatory and autoimmune properties. The concentration of bilirubin in human body is related to sex, drug intake, age and so on. Low serum bilirubin is directly related to diabetes, metabolic syndrome, cardiovascular disease and other pathological states. However, high bilirubin is indicative of hemolysis, jaundice, Gilbert syndrome, hepatitis, drug toxicity, and possible bile duct obstruction.

Performance characteristics

Synonyms TBIL
Sample type Animal serum
Sensitivity 0.7 μmol/L
Detection range 0.7-50 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 4%Average intra-assay CV: 2.800%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Storage 2-8℃
Valid period 12 months

Images

The concentration of TBA was significantly changed in each group. (**P<0.01 vs Normal, #P<0.05, ##P<0.01 vs Model, △P<0.05, △△△P<0.001 vs High-dose)

BB Liu et al investigate the function of SHCZ in ameliorating liver injury. Total bilirubin (TBIL) in rat liver was determined using TBIL colorimetric assay kit (E-BC-K760-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.7-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

Mouse serum

1

Rabbit serum

1

Chicken serum

1

Porcine serum

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

  1. Cells (2021) IF: 6.6
    Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis

    DOI: 10.3390/cells10071622

    PMID: 34209524

    Sample: serum
  2. Nutrients (2023) IF: 6.706
    Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets

    DOI: 10.3390/nu15040832

    Sample: serum
  3. Cells (2019) IF: 5.656
    The Role of GLP1 in Rat Steatotic and Non-Steatotic Liver Transplantation from Cardiocirculatory Death Donors

    DOI: 10.3390/cells8121599

    Sample: plasma
  4. American Journal of Translational Research (2021) IF: 4.06
    Personalized nursing improves physical condition and life quality of patients undergoing interventional therapy for liver cancer

    PMID: 35035768

    Sample: Blood
  5. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540

    Sample: hepatic tissue,serum
  6. Journal of Oleo Science (2022) IF: 1.628
    Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

    DOI: 10.5650/jos.ess22033

    PMID: 35965085

    Sample: serum
  7. DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742
    Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

    DOI: 10.1002/ddr.21581

    Sample: liver
  8. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002

    Sample: plasma
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