Total Bilirubin (TBIL) Colorimetric Assay Kit (E-BC-K760-M)
For research use only.
| Detection Principle | Under the action of accelerant, the hydrogen bond in indirect bilirubin is broken, which makes the insoluble indirect bilirubin and direct bilirubin react with azo reagent to form azo bilirubin under acidic conditions. The azo bilirubin generated has the maximum absorption at 565 nm. The content of total bilirubin in serum can be obtained by measuring the change of absorbance. |
| Synonyms | TBIL |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (565 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.7 μmol/L |
| Detection Range | 0.7-50 μmol/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 2.8% |
| Sample Volume | 60 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Rabbit serum | 1 |
| Chicken serum | 1 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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