Current Position:Home>>Kits>>Metabolism Assays>> Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)
Cat.No.:E-BC-K109-M
Welcome to order from local distributors.
Protocols for metabolism assays
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Synonyms | TC |
Sample type | Serum,plasma,tissue |
Sensitivity | 0.29 mmol/L |
Detection range | 0.29-25.85 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 8.300%Average intra-assay CV: 3.100% |
Other instruments required | Micropipettor, Incubator, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Anhydrous ethanol |
Storage | 2-8℃ |
Valid period | 12 months |
The concentration of cholesterol in THP-1 cell was significantly increased in ox-LDL group. (***P<0.001)
C Zhu et al found that TRIM64 can promote pyroptosis, foam cell formation and inflammation by activating NF-κB. Total cholesterol (TC) levels in human THP-1 cell was determined using TC colorimetric assay kit (E-BC-K109-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-25.85 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
Dilution factor
Human serum
1
Mouse serum
1
Rat plasma
1
10% Mouse liver tissue homogenate
1
10% Mouse kidney tissue homogenate
1
10% Mouse heart tissue homogenate
1
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples.
PMID: 36808719 |
Sample: Serum |
DOI: 10.1111/cpr.13332 |
|
Sample: liver |
Sample: Serum |
DOI: 10.1039/D2FO03509J |
|
Sample: serum |
PMID: 33784603 |
Sample: serum |
Sample: plasma |
DOI: 10.3390/nu14214665 |
|
Sample: liver |
PMID: 36229750 |
Sample: THP-1 cells |
Sample: serum |
PMID: 34438294 |
Sample: plasma |
Sample: Serum |
Sample: serum,liver |
PMID: 34733235 |
Sample: serum |
PMID: 36230124 |
Sample: liver,fecal,serum |
PMID: 35963464 |
Sample: plasma |
PMID: 35967043 |
Sample: serum |
PMID: 35151834 |
Sample: serum |
PMID: 34252510 |
Sample: serum |
Sample: blood |
DOI: 10.1111/jcmm.15498 |
|
Sample: Serum |
PMID: 35429740 |
Sample: serum |
DOI: 10.2147/DMSO.S348800 |
|
PMID: 35140491 |
Sample: serum |
Sample: plasma |
Sample: serum |
DOI: 10.1111/are.15474 |
|
Sample: serum |
PMID: 32980686 |
Sample: Serum |
PMID: 33590347 |
Sample: Tissue homogenate(liver) |
Sample: plasma |
Sample: liver tissue |
PMID: 31938118 |
Sample: serum |
Sample: plasma |
Sample: Plasma |
PMID: 32641048 |
Sample: Serum,Tissue homogenate |
PMID: 32848317 |
Sample: Serum |
Sample: serum |
Sample: serum |
M*****aSubmitted [ Oct 10 2022 ]
Asked: The homogenate medium of TC described in the protocol is isopropanol. Can it be changed to normal saline?`
Reply
adminSubmitted [ Oct 10 2022 ]
Answered: I really don't recommend that, the effect of normal saline homogenate is not good, leading to undetectable results.
M*****aSubmitted [ Sep 27 2022 ]
Asked: The homogenate medium of TC described in the protocol is isopropanol. Can it be changed to normal saline?`
Reply
adminSubmitted [ Sep 27 2022 ]
Answered: I really don't recommend that, the effect of normal saline homogenate is not good, leading to undetectable results.
M*****aSubmitted [ Sep 08 2022 ]
Asked: Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method) CAT. No.:E-BC-K109-M, Could this Kit be used for the detection of bacterial fluid?
Reply
adminSubmitted [ Sep 08 2022 ]
Answered: The sample is special and we have not verified it. If the sample content is higher than the sensitivity of the kit, it can be detected.