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Total Iron Colorimetric Assay Kit

Cat.No.:E-BC-K772-M
Detection instrument: Microplate reader (590-600 nm, optimum wavelength: 593 nm）

Detection method: Colorimetric method

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Detection principle

Under the action of reductant, iron ions in samples can be reduced into ferrous ions (Fe2+). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.

Performance characteristics

Sample type	serum, tissue
Sensitivity	0.4 μmol/L
Detection range	0.4-50 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	80min
Precision	Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Storage	2-8℃
Valid period	12 months

Images

The increased iron content in 4T1 cells showed significantly difference between different treatments.

H Tian et al developed a targeted nanomodulator that can induced tumor cell ferroptosis. Iron (Fe) level in mouse tumor cells 4T1 were determined using total iron colorimetric assay kit (E-BC-K772-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.4-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1-2
Rat serum	1
10% Mouse liver tissue homogenate	2-3
10% Rat lung tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	2-3
10% Epipremnum aureum leaf tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

PHYTOMEDICINE (2022) IF: 6.656
Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress

DOI: 10.1016/j.phymed.2022.154330

PMID: 35905566
Sample: chondrocytes
ENVIRONMENTAL TOXICOLOGY (2023) IF: 4.109
GPX4 utilization by selenium is required to alleviate cadmium-induced ferroptosis and pyroptosis in sheep kidney

DOI: 10.1002/tox.23740

Sample: Kidney
BIOLOGY OF REPRODUCTION (2021) IF: 4.285
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

DOI: 10.1093/biolre/ioab040

Sample: serum，ovary
Frontiers in Veterinary Science (2021) IF: 3.412
Deletion of the c2515 and c2516 Genes Affects Iron Uptake and Virulence of APEC O1 Strain E516

DOI: 10.3389/fvets.2021.654721

PMID: 33912608
Sample: Cell sample,Tissue homogenate

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