Ferrous Iron Colorimetric Assay Kit (E-BC-K773-M)
For research use only.
| Detection Principle | Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly. |
| Detection Method | Colorimetric method |
| Sample Type | Serum;Tissue |
| Assay Time | 40 min |
| Detection Instrument | Microplate reader |
| Ex/Em | Microplate reader (590-600 nm) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1-2 |
| Rat serum | 1 |
| 10% Mouse liver tissue homogenate | 2-3 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 2-3 |
| 10% Epipremnum aureum leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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