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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Name: Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
Brand: Elabscience
SKU: E-BC-K020-M
Price: 260 USD
Availability: InStock
Rating: 5 (3 reviews)

Citations(13)Rating(4.7)

Catalog number:E-BC-K020-M

Size:

96T
48T
500Assays

Qty:

- +

Price: $260

Can detect 92 samples if without duplication

Detection method: Colorimetric method

Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

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Datasheet
Citations(13)
Reviews(3)
Q&A

Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Performance characteristics

Synonyms	T-SOD
Sample type	Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity	0.2 U/mL
Detection range	0.2 -14.4 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
Other instruments required	Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃, others: 2-8℃
Valid period	12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	3-5
Rat serum	20-30
Urine	1
Human hydrothorax	2
Cell culture supernatant	2-3
10% Rat liver tissue homogenate	340-370
10% Rat heart tissue homogenate	80-100
10% Rat kidney tissue homogenate	100-120
10% Rat brain tissue homogenate	50-100
10% Plant tissue homogenate	5-10
HepG2 cells (3 mgprot/mL)	30-40

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Related Products

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E-BC-K031-M	Catalase (CAT) Activity Assay Kit
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Citations

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress
IF: 4.694
Journal: Biomolecules(2019)
DOI: 10.3390/biom9090453
PMID: 31489882
Products: E-BC-K020-M
Sample type: Tissue homogenate
Reactivity: Rat
2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish
IF: 3.743
Journal: BIOMEDICINE & PHARMACOTHERAPY(2020)
DOI: 10.1016/j.biopha.2020.110138
PMID: 99999999
Products: E-BC-K013-M E-BC-K020-M E-EL-0060
Sample type: Tissue homogenate
Reactivity: Fish
Determination of Half Maximal Inhibitory Concentration of CaAl Layered Double Hydroxide on Cancer Cells and Its Role in the Apoptotic Pathway
IF: 3.89
Journal: Applied Clay Science(2019)
DOI: 10.1016/j.clay.2018.10.013
PMID: 99999999
Products: E-BC-K020-M
Sample type: HCT 116 cells
Reactivity: Human
Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension
IF: 3.17
Journal: European Journal of Pharmacology(2019)
DOI: 10.1016/j.ejphar.2019.01.059
PMID: 30716317
Products: E-BC-K020-M E-BC-K031-S E-BC-K096-S
Sample type: Tissue homogenate
Reactivity: Rat
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
IF: 2.518
Journal: Biomedical and Environmental Sciences(2018)
DOI: 10.3967/bes2018.101
PMID: 30423277
Products: E-BC-K035-M E-BC-K020-M
Sample type: Plasma,Tissue homogenate
Reactivity: Mouse
The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.
IF: 3.04
Journal: Journal of Diabetes Research(2018)
DOI: 10.1155/2018/7341242
PMID: 30009183
Products: E-BC-K096-S E-BC-K020-M
Sample type: Tissue homogenate
Reactivity: Rat
Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size
IF: 1.175
Journal: Pakistan Veterinary Journal(2019)
DOI: 10.29261/pakvetj/2019.106
PMID: 99999999
Products: E-BC-K020-M
Sample type: Tissue homogenate
Reactivity: Rat
Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract
Journal: African Journal of Pharmacy and Pharmacology(2019)
DOI: 10.5897/AJPP2019.5090
PMID: 99999999
Products: E-BC-K020-M E-BC-K031-M
Sample type: Serum
Reactivity: Rat
Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city
Journal: JOURNAL OF EDUCATION AND SCIENCE(2020)
DOI: 10.33899/edusj.2020.126325.1031
PMID: 99999999
Products: E-BC-K020-M E-BC-K097-M
Sample type: Serum
Reactivity: Human
Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
Journal: Journal of International Pharmaceutical Research(2019)
DOI: 99999999
PMID: 99999999
Products: E-BC-K020-M E-BC-K022-M E-BC-K025-M E-BC-K029-S E-BC-K030-M E-BC-K096-M
Sample type: Serum
Reactivity: Rabbit
The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
IF: 0.24
Journal: Latin American Journal of Pharmacy(2019)
DOI: 99999999
PMID: 99999999
Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S
Sample type: Serum,Tissue homogenate
Reactivity: Mouse
Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
IF: 0.395
Journal: Indian Journal of Animal Research(2019)
DOI: 10.18805/ijar.B-1157
PMID: 99999999
Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M
Sample type: Tissue homogenate
Reactivity: Mussel
Effect of alcohol stress on cell growth and its recovery of Synechocystis spPCC 6803.
IF: /
Products: E-BC-K020-M
Sample type: Synechocystis sp. PCC 6803 wild type
Reactivity: Synechocystis sp.

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Reviews (3)
Q&A (0)

Verified Customer

l**pSubmitted [ Jun 06 2019 ]

Application:Biochemical
Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.

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Verified Customer

s***rSubmitted [ May 31 2019 ]

Application:Biochemical
Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity

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Verified Customer

M***ySubmitted [ May 31 2019 ]

Application:Biochemical
Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well

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