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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Name: Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
Brand: Elabscience
SKU: E-BC-K020-M
Price: 260 USD
Availability: InStock
Rating: 5 (3 reviews)

Citations(12)

Cat.No.:E-BC-K020-M
Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Performance characteristics

Synonyms	T-SOD
Sample type	Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity	0.2 U/mL
Detection range	0.2 -14.4 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
Other instruments required	Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃, others: 2-8℃
Valid period	12 months

Images

The activity of SOD increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced SOD activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Total superoxide dismutase (T-SOD) activity of fungal spore was determined using T-SOD activity assay kit (E-BC-K020-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	3-5
Rat serum	20-30
Urine	1
Human hydrothorax	2
Cell culture supernatant	2-3
10% Rat liver tissue homogenate	340-370
10% Rat heart tissue homogenate	80-100
10% Rat kidney tissue homogenate	100-120
10% Rat brain tissue homogenate	50-100
10% Plant tissue homogenate	5-10
HepG2 cells (3 mgprot/mL)	30-40

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Ralated product

Cat Number	Product Name
E-BC-K031-M	Catalase (CAT) Activity Assay Kit
E-BC-K025-M	Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Biomolecules (2019) IF: 4.694

Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress

DOI: 10.3390/biom9090453
PMID: 31489882	Sample: Tissue homogenate

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 3.743

2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

DOI: 10.1016/j.biopha.2020.110138
	Sample: Tissue homogenate

Applied Clay Science (2019) IF: 3.89

Determination of Half Maximal Inhibitory Concentration of CaAl Layered Double Hydroxide on Cancer Cells and Its Role in the Apoptotic Pathway

DOI: 10.1016/j.clay.2018.10.013
	Sample: HCT 116 cells

European Journal of Pharmacology (2019) IF: 3.17

Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

DOI: 10.1016/j.ejphar.2019.01.059
PMID: 30716317	Sample: Tissue homogenate

Biomedical and Environmental Sciences (2018) IF: 2.518

Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

DOI: 10.3967/bes2018.101
PMID: 30423277	Sample: Plasma,Tissue homogenate

Journal of Diabetes Research (2018) IF: 3.04

The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.

DOI: 10.1155/2018/7341242
PMID: 30009183	Sample: Tissue homogenate

Pakistan Veterinary Journal (2019) IF: 1.175

Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size

DOI: 10.29261/pakvetj/2019.106
	Sample: Tissue homogenate

JOURNAL OF EDUCATION AND SCIENCE (2020)

Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city

DOI: 10.33899/edusj.2020.126325.1031
	Sample: Serum

Journal of International Pharmaceutical Research (2019)

Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits


	Sample: Serum

Latin American Journal of Pharmacy (2019) IF: 0.24

The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice


	Sample: Serum,Tissue homogenate

Indian Journal of Animal Research (2019) IF: 0.395

Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

DOI: 10.18805/ijar.B-1157
	Sample: Tissue homogenate

IF: /

Effect of alcohol stress on cell growth and its recovery of Synechocystis spPCC 6803.


	Sample: Synechocystis sp. PCC 6803 wild type

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Reviews/Q&A

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Reviews
Q&A

Verified Customer

l**pSubmitted [ Jun 06 2019 ]

Application:Biochemical
Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.

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Verified Customer

s***rSubmitted [ May 31 2019 ]

Application:Biochemical
Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity

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Verified Customer

M***ySubmitted [ May 31 2019 ]

Application:Biochemical
Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well

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M*****aSubmitted [ Jul 28 2022 ]

Asked: Hello, I measured the SOD inhibition rate with liver tissue homogenate is about 80%, how many times should be diluted?

adminSubmitted [ Jul 28 2022 ]

Answered: It indicates that the dilution is still too low. It is recommended to increase the dilution about 200-300 times.

M*****aSubmitted [ Jul 28 2022 ]

Asked: What is the dilution factor of the mouse serum?

adminSubmitted [ Jul 28 2022 ]

Answered: It is recommended to dilute for 20 times.

N*rSubmitted [ Jul 19 2022 ]

Asked: I have problem with the calculation of inhibition ratio of SOD. What does Acontrol and Ablank control means? Where can I get the figures? Thank you

adminSubmitted [ Jul 19 2022 ]

Answered: If there are no samples added for the control hole and blank control. The corresponding OD value can be obtained after operation according to the instructions, which can be used for calculation.

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