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Current Position:Home>>Kits>>Metabolism Assays>> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Citations(12)Uniprot : ml,yhyj

  • Cat.No.:E-BC-K020-M

  • Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

Manual MSDS

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Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity 0.2 U/mL
Detection range 0.2 -14.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
Other instruments required Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

3-5

Rat serum

20-30

Urine

1

Human hydrothorax

2

Cell culture supernatant

2-3

10% Rat liver tissue homogenate

340-370

10% Rat heart tissue homogenate

80-100

10% Rat kidney tissue homogenate

100-120

10% Rat brain tissue homogenate

50-100

10% Plant tissue homogenate

5-10

HepG2 cells (3 mgprot/mL)

30-40

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomolecules (2019) IF: 4.694
    Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress

    DOI: 10.3390/biom9090453

    PMID: 31489882

    		 Sample: Tissue homogenate
  2. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 3.743
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138

    PMID: 99999999

    		 Sample: Tissue homogenate
  3. Applied Clay Science (2019) IF: 3.89
    Determination of Half Maximal Inhibitory Concentration of CaAl Layered Double Hydroxide on Cancer Cells and Its Role in the Apoptotic Pathway

    DOI: 10.1016/j.clay.2018.10.013

    PMID: 99999999

    		 Sample: HCT 116 cells
  4. European Journal of Pharmacology (2019) IF: 3.17
    Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

    DOI: 10.1016/j.ejphar.2019.01.059

    PMID: 30716317

    		 Sample: Tissue homogenate
  5. Biomedical and Environmental Sciences (2018) IF: 2.518
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

    DOI: 10.3967/bes2018.101

    PMID: 30423277

    		 Sample: Plasma,Tissue homogenate
  6. Journal of Diabetes Research (2018) IF: 3.04
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.

    DOI: 10.1155/2018/7341242

    PMID: 30009183

    		 Sample: Tissue homogenate
  7. Pakistan Veterinary Journal (2019) IF: 1.175
    Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size

    DOI: 10.29261/pakvetj/2019.106

    PMID: 99999999

    		 Sample: Tissue homogenate
  8. JOURNAL OF EDUCATION AND SCIENCE (2020)
    Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city

    DOI: 10.33899/edusj.2020.126325.1031

    PMID: 99999999

    		 Sample: Serum
  9. Journal of International Pharmaceutical Research (2019)
    Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits

    PMID: 99999999

    		 Sample: Serum
  10. Latin American Journal of Pharmacy (2019) IF: 0.24
    The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice

    PMID: 99999999

    		 Sample: Serum,Tissue homogenate
  11. Indian Journal of Animal Research (2019) IF: 0.395
    Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

    DOI: 10.18805/ijar.B-1157

    PMID: 99999999

    		 Sample: Tissue homogenate
  12. IF: /
    Effect of alcohol stress on cell growth and its recovery of Synechocystis spPCC 6803.
    Sample: Synechocystis sp. PCC 6803 wild type
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Reviews/Q&A

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  • Reviews
  • Q&A

Verified Customer

l**pSubmitted [ Jun 06 2019 ]

  • Application:Biochemical
  • Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.
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Verified Customer

s***rSubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity
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Verified Customer

M***ySubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well
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