Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K020-M

      Size:
      • 96T
      • 48T
      • 500Assays
      Qty:
      - +
      Price: $260
      Can detect 92 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Detection principle

      The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

      Performance characteristics

      Synonyms T-SOD
      Sample type Serum,plasma,hydrothorax,ascites,urine,cells,tissue
      Sensitivity 0.2 U/mL
      Detection range 0.2 -14.4 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 30 min
      Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
      Other instruments required Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
      Storage Reagent 3: -20℃, others: 2-8℃
      Valid period 12 months

      Dilution of sample

      The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      3-5

      Rat serum

      20-30

      Urine

      1

      Human hydrothorax

      2

      Cell culture supernatant

      2-3

      10% Rat liver tissue homogenate

      340-370

      10% Rat heart tissue homogenate

      80-100

      10% Rat kidney tissue homogenate

      100-120

      10% Rat brain tissue homogenate

      50-100

      10% Plant tissue homogenate

      5-10

      HepG2 cells (3 mgprot/mL)

      30-40

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress
        IF: 4.694
        Journal: Biomolecules(2019)
        DOI: 10.3390/biom9090453
        PMID: 31489882
        Products: E-BC-K020-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. 2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish
        IF: 3.743
        Journal: BIOMEDICINE & PHARMACOTHERAPY(2020)
        DOI: 10.1016/j.biopha.2020.110138
        PMID: 99999999
        Products: E-BC-K013-M E-BC-K020-M E-EL-0060 
        Sample type: Tissue homogenate
        Reactivity: Fish
      3. Determination of Half Maximal Inhibitory Concentration of CaAl Layered Double Hydroxide on Cancer Cells and Its Role in the Apoptotic Pathway
        IF: 3.89
        Journal: Applied Clay Science(2019)
        DOI: 10.1016/j.clay.2018.10.013
        PMID: 99999999
        Products: E-BC-K020-M 
        Sample type: HCT 116 cells
        Reactivity: Human
      4. Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension
        IF: 3.17
        Journal: European Journal of Pharmacology(2019)
        DOI: 10.1016/j.ejphar.2019.01.059
        PMID: 30716317
        Products: E-BC-K020-M E-BC-K031-S E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      5. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
        IF: 2.518
        Journal: Biomedical and Environmental Sciences(2018)
        DOI: 10.3967/bes2018.101
        PMID: 30423277
        Products: E-BC-K035-M E-BC-K020-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
      6. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.
        IF: 3.04
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K096-S E-BC-K020-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      7. Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size
        IF: 1.175
        Journal: Pakistan Veterinary Journal(2019)
        DOI: 10.29261/pakvetj/2019.106
        PMID: 99999999
        Products: E-BC-K020-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      8. Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract
        Journal: African Journal of Pharmacy and Pharmacology(2019)
        DOI: 10.5897/AJPP2019.5090
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K031-M 
        Sample type: Serum
        Reactivity: Rat
      9. Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city
        Journal: JOURNAL OF EDUCATION AND SCIENCE(2020)
        DOI: 10.33899/edusj.2020.126325.1031
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K097-M 
        Sample type: Serum
        Reactivity: Human
      10. Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
        Journal: Journal of International Pharmaceutical Research(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K022-M E-BC-K025-M E-BC-K029-S E-BC-K030-M E-BC-K096-M 
        Sample type: Serum
        Reactivity: Rabbit
      11. The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
        IF: 0.24
        Journal: Latin American Journal of Pharmacy(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      12. Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study
        IF: 0.395
        Journal: Indian Journal of Animal Research(2019)
        DOI: 10.18805/ijar.B-1157
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-M E-BC-K030-M E-BC-K031-M E-BC-K096-M 
        Sample type: Tissue homogenate
        Reactivity: Mussel
      13. Effect of alcohol stress on cell growth and its recovery of Synechocystis spPCC 6803.
        IF: /
        Products: E-BC-K020-M 
        Sample type: Synechocystis sp. PCC 6803 wild type
        Reactivity: Synechocystis sp.
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      • Reviews (3)
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      Verified Customer

      l**pSubmitted [ Jun 06 2019 ]

      • Application:Biochemical
      • Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.
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      Verified Customer

      s***rSubmitted [ May 31 2019 ]

      • Application:Biochemical
      • Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity
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      Verified Customer

      M***ySubmitted [ May 31 2019 ]

      • Application:Biochemical
      • Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well
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