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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

  • Cat.No.:E-BC-K020-M

  • Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)

To Purchase E-BC-K020-M

  • 96T
  • 48T
  • 500Assays
Price: $260

Detection principle

The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,hydrothorax,ascites,urine,cells,tissue
Sensitivity 0.2 U/mL
Detection range 0.2 -14.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.9%
Other instruments required Micropipettor, Multichannel pipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 12 months


The activity of SOD increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced SOD activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Total superoxide dismutase (T-SOD) activity of fungal spore was determined using T-SOD activity assay kit (E-BC-K020-M).

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum




Human hydrothorax


Cell culture supernatant


10% Rat liver tissue homogenate


10% Rat heart tissue homogenate


10% Rat kidney tissue homogenate


10% Rat brain tissue homogenate


10% Plant tissue homogenate


HepG2 cells (3 mgprot/mL)


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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  1. Biomolecules (2019) IF: 4.694
    Curcumin Attenuates Lead-Induced Cerebellar Toxicity in Rats via Chelating Activity and Inhibition of Oxidative Stress

    DOI: 10.3390/biom9090453

    PMID: 31489882

    Sample: Tissue homogenate
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138

    Sample: Tissue homogenate
  3. Applied Clay Science (2019) IF: 3.89
    Determination of Half Maximal Inhibitory Concentration of CaAl Layered Double Hydroxide on Cancer Cells and Its Role in the Apoptotic Pathway

    DOI: 10.1016/j.clay.2018.10.013

    Sample: HCT 116 cells
  4. European Journal of Pharmacology (2019) IF: 3.17
    Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

    DOI: 10.1016/j.ejphar.2019.01.059

    PMID: 30716317

    Sample: Tissue homogenate
  5. Biomedical and Environmental Sciences (2018) IF: 2.518
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

    DOI: 10.3967/bes2018.101

    PMID: 30423277

    Sample: Plasma,Tissue homogenate
  6. Journal of Diabetes Research (2018) IF: 3.04
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.

    DOI: 10.1155/2018/7341242

    PMID: 30009183

    Sample: Tissue homogenate
  7. Pakistan Veterinary Journal (2019) IF: 1.175
    Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size

    DOI: 10.29261/pakvetj/2019.106

    Sample: Tissue homogenate
    Physiological sway of aging on oxidative stress and levels of some minerals in diabetes type II of both female and male in Sulaimaniyah city

    DOI: 10.33899/edusj.2020.126325.1031

    Sample: Serum
  9. Journal of International Pharmaceutical Research (2019)
    Effect of the Alcoholic or the Aqueous Extracts of Ziziphus Spina-christi Leaves on the White Blood Cells and the Oxidative Stress Status in Local Rabbits
    Sample: Serum
  10. Latin American Journal of Pharmacy (2019) IF: 0.24
    The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
    Sample: Serum,Tissue homogenate
  11. Indian Journal of Animal Research (2019) IF: 0.395
    Histopathological and Biochemical Biomarker Response of Mussel, Unio Pictorum, to Carbamate Pesticide Carbaryl: A Laboratory Study

    DOI: 10.18805/ijar.B-1157

    Sample: Tissue homogenate
  12. IF: /
    Effect of alcohol stress on cell growth and its recovery of Synechocystis spPCC 6803.
    Sample: Synechocystis sp. PCC 6803 wild type
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  • Reviews
  • Q&A

Verified Customer

l**pSubmitted [ Jun 06 2019 ]

  • Application:Biochemical
  • Description:This kit has few sampling procedures, convenient operation, reasonable detection time, and the experimental results are clear and accurate, but the inhibition rate of this kit is difficult to grasp. When the dilution multiple of some samples reaches, the other part is on the high side or on the low side. If there are ways to improve or replace in this respect, the kit will be perfect.
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Verified Customer

s***rSubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample I tested was mouse serum,reference dilution given according to instructions, the kit need very little sample volume,besides, SOD kit easy to use and high sensitivity
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Verified Customer

M***ySubmitted [ May 31 2019 ]

  • Application:Biochemical
  • Description:The sample needs to be pre-tested, but the instructions provide dilutions range of the different sample. In general, the kit performs well
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M*****aSubmitted [ Jul 28 2022 ]

Asked: Hello, I measured the SOD inhibition rate with liver tissue homogenate is about 80%, how many times should be diluted?



adminSubmitted [ Jul 28 2022 ]

Answered: It indicates that the dilution is still too low. It is recommended to increase the dilution about 200-300 times.


M*****aSubmitted [ Jul 28 2022 ]

Asked: What is the dilution factor of the mouse serum?



adminSubmitted [ Jul 28 2022 ]

Answered: It is recommended to dilute for 20 times.


N*rSubmitted [ Jul 19 2022 ]

Asked: I have problem with the calculation of inhibition ratio of SOD. What does Acontrol and Ablank control means? Where can I get the figures? Thank you



adminSubmitted [ Jul 19 2022 ]

Answered: If there are no samples added for the control hole and blank control. The corresponding OD value can be obtained after operation according to the instructions, which can be used for calculation.

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