Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) (E-BC-K020-M)
For research use only.
| Detection Principle | The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products. |
| Detection Principle Image | |
| Synonyms | T-SOD |
| Sample Type | Serum,Plasma,Hydrothorax,ascites,Urine,Cell,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.2 U/mL |
| Detection Range | 0.2-14.4 U/mL |
| Precision | inter-assay CV: 3.7% | intra-assay CV: 2.9% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 3-5 |
| Rat serum | 20-30 |
| Urine | 1 |
| Human hydrothorax | 2 |
| Cell culture supernatant | 2-3 |
| 10% Rat liver tissue homogenization | 340-370 |
| 10% Rat heart tissue homogenization | 80-100 |
| 10% Rat kidney tissue homogenization | 100-120 |
| 10% Rat brain tissue homogenization | 50-100 |
| HepG2 cells homogenization (3 mgprot/mL) | 30-40 |
| 10% Plant tissue homogenization | 5-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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