Current Position:Home>>Kits>>Biochemical Kits>> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
Catalog number:E-BC-K020-M
Detection method: Colorimetric method
Detection instrument: Microplate reader(440-460 nm, optimum wavelength: 450 nm)
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Add to cart Compare Bulk requestSynonyms | T-SOD |
Sample type | Serum,plasma,hydrothorax,ascites,urine,cells,tissue |
Sensitivity | 0.2 U/mL |
Detection range | 0.2 -14.4 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 30 min |
Precision | Average inter-assay CV: 3.7%Average intra-assay CV: 2.9% |
Other instruments required | Micropipettor, Multichannel pipettor, Vortex mixer, Incubator |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | Reagent 3: -20℃, others: 2-8℃ |
Valid period | 6 months |
The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 25%~65% (the optimal inhibition ratio is the range of 40%~60%.) before formal experiment.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
3-5 |
Rat serum |
20-30 |
Urine |
1 |
Human hydrothorax |
2 |
Cell culture supernatant |
2-3 |
10% Rat liver tissue homogenate |
340-370 |
10% Rat heart tissue homogenate |
80-100 |
10% Rat kidney tissue homogenate |
100-120 |
10% Rat brain tissue homogenate |
50-100 |
10% Plant tissue homogenate |
5-10 |
HepG2 cells (3 mgprot/mL) |
30-40 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
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