When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

General Information

Application	DNA Fragmentation
Detection method	HRP-DAB
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Light microscope
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene，Ethanol，DNase I，PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120)，Neutral gum(E-IR-R118)
Storage	2~8°C/-20°C, shading light. Refer to the Manual.
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A311
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Citations

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Biological Trace Element Research (2020) IF: 2.639

In Vitro and In Vivo Pretreatment with Selenium Mitigates Tetrahydrocannabinol-Induced Testicular Cell Apoptosis: the Role of AKT and p53 Pathways

DOI: 10.1007/s12011-020-02322-5
PMID: 32815089	Sample: Cell sample

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