TUNEL In Situ Apoptosis Kit (HRP-DAB Method) (E-CK-A331)
For research use only.
| Detection Principle | When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis. |
| Detection Method | Colorimetric method |
| Sample Type | Paraffin section;frozen section;cell slide |
| Assay Time | 3 hours |
| Detection Instrument | Light microscope |
| Other Reagents Required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene，Ethanol，DNase I，PBS(E-BC-R187),ddH2O,Hematoxylin(E-IR-R120)，Neutral gum(E-IR-R118) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
