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TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

  • Cat.No.:E-CK-A331

To Purchase E-CK-A331

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Price: $280

Detection principle

When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

General Information

Application DNA Fragmentation
Detection method HRP-DAB
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Light microscope
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene,Ethanol,DNase I,PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120),Neutral gum(E-IR-R118)
Storage 2~8°C/-20°C, shading light. Refer to the Manual.
Shipping Ice bag
Expiration date 12 months


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biological Trace Element Research (2020) IF: 2.639
    In Vitro and In Vivo Pretreatment with Selenium Mitigates Tetrahydrocannabinol-Induced Testicular Cell Apoptosis: the Role of AKT and p53 Pathways

    DOI: 10.1007/s12011-020-02322-5

    PMID: 32815089

    Sample: Cell sample


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