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Cat.No.:E-CK-A331
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When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.
Application | DNA Fragmentation |
Detection method | HRP-DAB |
Sample type | Paraffin section, frozen section, cell slide |
Assay time | 3 hours |
Detection instrument | Light microscope |
Other reagents required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene,Ethanol,DNase I,PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120),Neutral gum(E-IR-R118) |
Storage | 2~8°C/-20°C, shading light. Refer to the Manual. |
Shipping | Ice bag |
Expiration date | 12 months |
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PMID: 32815089 |
Sample: Cell sample |