Uncoated Chicken Cort（Corticosterone） ELISA Kit (E-UNEL-Ch0002)

Name: Uncoated Chicken Cort（Corticosterone） ELISA Kit
Brand: Elabscience
SKU: E-UNEL-Ch0002
Price: 260.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Chicken Cort（Corticosterone） ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	3.125-200ng/mL
Sample Volume	50μL
Total Assay Time	24 h
Reactivity	Chicken
Specificity	This kit recognizes Chicken Cort in samples.No significant cross-reactivity or interference between Chicken Cort and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
200	0.108	0.108	0.108	-
100	0.151	0.156	0.154	-
50	0.218	0.212	0.215	-
25	0.387	0.361	0.374	-
12.5	0.678	0.639	0.659	-
6.25	1.082	1.049	1.066	-
3.125	1.675	1.551	1.613	-
0	2.556	2.396	2.476	-

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken Cortisol are added to the micro ELISA plate wells. Chicken Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Chicken Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.