Uncoated Human CD276 (Cluster of Differentiation 276) ELISA Kit (E-UNEL-H0244)

Name: Uncoated Human CD276 (Cluster of Differentiation 276) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0244
Price: 399.0 USD
Availability: InStock

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Uncoated Human CD276 (Cluster of Differentiation 276) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human CD276 in samples.No significant cross-reactivity or interference between Human CD276 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	2.303	2.267	2.285	2.191
5	1.483	1.509	1.496	1.402
2.5	0.953	0.967	0.960	0.866
1.25	0.484	0.468	0.476	0.382
0.63	0.323	0.317	0.320	0.226
0.31	0.192	0.192	0.192	0.098
0.16	0.143	0.151	0.147	0.053
0	0.094	0.094	0.094	-

Background

The protein encoded by this gene belongs to the immunoglobulin superfamily; and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors; the protein is preferentially expressed only in tumor tissues. Additionally; it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA; and there is an inverse correlation between the expression of this protein and miR29 levels; suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.