Uncoated Human DEFβ2/DEFB2 (Defensin Beta 2) ELISA Kit (E-UNEL-H0284)
Product Summary
The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
| Detection Range | 62.5-4000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 24 h |
| Reactivity | Human |
| Specificity | This kit recognizes Human DEFβ2/DEFB2 in samples.No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T*5 / 96T*15 |
| Storage | -20℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
|
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. Prior to the assay, the plate must be coated with antibody specific for Human DEFβ2/DEFB2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.
Background
Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short; processed peptide molecules that are classified by structure into three groups: alpha-defensins; beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin; beta 104; DEFB104A and DEFB104B; in head-to-head orientation. This gene; DEFB104A; represents the more centromeric copy.
| Gene ID | 100289462 |
| Uniport ID | O15263 |
| Research Area | Cancer , Microbiology |
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