Uncoated Human F7 (Coagulation Factor Ⅶ) ELISA Kit (E-UNEL-H0314)

Name: Uncoated Human F7 (Coagulation Factor Ⅶ) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0314
Price: 399.0 USD
Availability: InStock

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Uncoated Human F7 (Coagulation Factor Ⅶ) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human F7 in samples.No significant cross-reactivity or interference between Human F7 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.258	2.336	2.297	2.218
2000	1.447	1.503	1.475	1.396
1000	0.799	0.755	0.777	0.698
500	0.479	0.505	0.492	0.413
250	0.276	0.258	0.267	0.188
125	0.175	0.165	0.170	0.091
62.5	0.131	0.127	0.129	0.05
0	0.083	0.075	0.079	-

Background

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.