Uncoated Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit (E-UNEL-H0073)

Name: Uncoated Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0073
Price: 260.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Uncoated Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 260.00	- +
	96T*15	$ 550.00	- +

Ancillary Products Available: Ancillary Reagent Kit

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Product Summary

The new series of Elabsciencee^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human OxLDL in samples.No significant cross-reactivity or interference between Human OxLDL and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.278	2.334	2.306	2.233
2000	1.454	1.454	1.454	1.381
1000	0.749	0.789	0.769	0.696
500	0.499	0.469	0.484	0.411
250	0.253	0.271	0.262	0.189
125	0.156	0.166	0.161	0.088
62.5	0.119	0.127	0.123	0.05
0	0.070	0.076	0.073	-

Background

OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.

Research Area

Cancer , Cardiovascular , Metabolism , Signaltransduction