Urea (BUN) Colorimetric Assay Kit (Urease Method)
Price: $ 150
Size:
96T
Quantity:
- Sample Type: serum;plasma;urine;saliva;milk
- Detection Instrument: Microplate reader(565-595 nm,optimum wavelength: 580 nm)
- Reasearch Areas: Liver Renal function biomarkers
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For research use only. Order now, ship in 3 days
Product Details
Detection Principle | Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with amphyl and form a green substance in alkaline medium, and the production of the green substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm. | ||||||||||
Synonyms | BUN | ||||||||||
Sample Type | serum,plasma,urine,saliva,milk | ||||||||||
Sensitivity | 0.09 mmol/L | ||||||||||
Detection Range | 0.28-35 mmol/L | ||||||||||
Detection Method | Colorimetric method | ||||||||||
Assay Time | 30 min | ||||||||||
Precision | inter-assay CV: 4.3% | intra-assay CV: 2.8% | ||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||
Valid Period | 12 months | ||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M,pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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