Urea Colorimetric Assay Kit (Diacetyl Oxime Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K329-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $150
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:

      Performance characteristics

      Synonyms BUN
      Sample type serum,plasma,saliva,urine
      Sensitivity 0.12 mmol/L
      Detection range 0.12-15 mmol/L
      Precision Average inter-assay CV: 9.9%Average intra-assay CV: 4.9%
      Other instruments required Vortex mixer, Micropipettor, Incubator, Water bath
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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