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Uric Acid (UA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K016-S

  • Detection instrument: Spectrophotometer(690 nm)

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Price: $180

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Protocols for metabolism assays

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Detection principle

Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.

Performance characteristics

Synonyms UA
Sample type Serum,plasma,urine
Sensitivity 0.58 mg/L
Detection range 0.58-100 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 2.600%Average intra-assay CV: 1.800%
Other instruments required Vortex mixer, Micropipettor, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months


Plasma UA shows no significant differences with different diets (P<0.05).

N A M Omar et al investigate the effect of high intakes of fructose and galactose, with or without added fructooligosaccharides (FOS), on metabolism factors, inflammation, and gut integrity markers in rats. Uric acid level in rat plasma was determined using UA colorimetric assay kit (E-BC-K016-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human urine


Human serum


Dog serum


Rat serum


Mouse serum


Porcine serum


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400

    Sample: plasma,urine
  2. Frontiers in Pharmacology (2021) IF: 5.811
    Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects.

    DOI: 10.3389/fphar.2021.772504

    Sample: Serum
  3. Drug Design Development and Therapy (2021) IF: 4.162
    Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

    DOI: 10.2147/DDDT.S326975

    PMID: 34703210

    Sample: serum
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Verified Customer

S***ySubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:The kit is generally good, easy and fast,My linear correlation coefficient of the standard curve is not to reach 0.99
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