ZEN (Zearalenone) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
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    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      Catalog number:E-TO-E002

      Size:
      • 96T
      Qty:
      - +
      Price: $240

      sample_type: Grain,Feed,Corn skin,Wheat bran

      Sensitivity: 0.3 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Zearalenone (ZEN) in samples, such as grain, feed and other samples. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The Microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, ZEN in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-ZEN antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of ZEN. The concentration of ZEN in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity: 0.3 ppb (ng/mL)

      Reaction mode: 25, 30min~ 15min

      Detection limit: Grain---6 ppb; Feed---18ppb; Corn skin, Wheat bran ---18 ppb

      Cross-reactivity: Zearalenone ---100%, Zearalanone ---13%, Zearanol ---<1%

      Sample recovery rate: Grain---90%±15%; Feed, Corn skin, Wheat bran----80%±15%

       


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid 

      1 mL each

      (0 ppb,0.3 ppb,0.9 ppb,2.7 ppb,8.1 ppb,24.3 ppb)

      HRP Conjugate 

      5.5 mL

      Antibody Working Solution 

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution 

      6 mL

      20×Concentrated Wash Buffer 

      40 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy




      Other supplies required

      Instrument: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility: 0.01g). 

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Methanol.



      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.

      .


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