4-HNE(4-Hydroxynonenal) ELISA Kit (E-EL-0128)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

An important link between elevated cytokines and oxidative stress during the initial inflammatory process changes the REDOX balance through a thiol-dependent mechanism, and HNE is the most active aldehydes produced during this process