Alpha-Glucosidase Inhibitor Screening Kit (E-BC-D017)
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For research use only.
| Detection Principle |
Alpha-glucosidase catalyzes the release of α-glucose from the non-reducing end of the substrate and promotes the absorption of glucose by the small intestine. Inhibition of α-glucosidase is an effective method for the treatment of non-insulin-dependent diabetes mellitus (NIDDM). The detection principle of this kit: α-Glucosidase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 400 nm. The addition of an inhibitor suppresses the enzymatic activity of α-glucosidase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference. |
| Sample Type | Inhibitor |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (390-410 nm, optimum wavelength: 400 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 5.6-8.6% | intra-assay CV: 0.2-2.0% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
| Cat.No. | Product Name | Clone No. |
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