Amylopectin Colorimetric Assay Kit (E-BC-K1201-M)
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For research use only.
| Detection Principle | Amylopectin can be combined with the chromogenic component at 530 nm for maximum absorption, and the dual-wavelength method can be used to determine the content of amylose more accurately. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (Detection wavelength: 530 nm; Reference wavelength: 710 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.012 mg/mL |
| Detection Range | 0.012-1mg/mL |
| Precision | inter-assay CV: 6.6-7.2% | intra-assay CV: 1.8-3.6% |
| Sample Volume | 50 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Potato tissue homogenate | 2-8 |
| 10% Corn tissue homogenate | 2-8 |
| 10% Rice tissue homogenate | 2-8 |
| 10% Edible starch tissue homogenate | 2-8 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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