Starch Debranching Enzyme (DBE) Activity Colorimetric Assay Kit (E-BC-K1206-M)
For research use only.
| Detection Principle | Starch Debranching Enzyme (DBE) can specifically split the α-1,6-glucoside bonds of amylopectin. The resulting reducing sugars were determined by 3,5-dinitrosalicylic acid method, and their light absorption values were measured at 540 nm. Then the activity of starch debranching enzyme was calculated indirectly. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.9 U/g |
| Detection Range | 0.9-18.0 U/g |
| Precision | inter-assay CV: 1.0-1.4% | intra-assay CV: 0.7-1.2% |
| Sample Volume | 120 μL(tissue homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Corn tissue homogenate | 2-8 |
| 10% Peanut tissue homogenate | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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