ATPase Activity Assay Kit (E-BC-K831-M)
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For research use only.
| Detection Principle | ATPase catalyzes the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and inorganic phosphorus. The activity of ATPase was determined by inorganic phosphorus production per unit time. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (630-650 nm, optimum wavelength: 640 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 U/L |
| Detection Range | 0.06-2.75 U/L |
| Precision | inter-assay CV: 5% | intra-assay CV: 4% |
| Sample Volume | 200 μL(tissue/cell homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 5-10 |
| 10% Rat kidney tissue homogenate | 5-10 |
| 10% Rat heart tissue homogenate | 5-10 |
| 10% Rat spleen tissue homogenate | 5-10 |
| 10% Rat lung tissue homogenate | 5-10 |
| 10% Mouse liver tissue homogenate | 5-10 |
| 10% Mouse spleen tissue homogenate | 5-10 |
| 10% Mouse lung tissue homogenate | 5-10 |
| 10% Mouse kidney tissue homogenate | 5-10 |
| 293T cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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