cAMP(Cyclic adenosine monophosphate) ELISA Kit (E-EL-0056)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal cAMP. During the reaction, Universal cAMP in the sample or standard competes with a fixed amount of Universal cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

The second messenger 3′,5′-cyclic adenosine monophosphate (cAMP) is involved in multiple physiological functions. In response to a variety of different hormones and other extracellular cues that operate via activation of G protein-coupled receptors (GPCRs), cAMP affects cellular processes that range from modulation of metabolic enzymes to regulation of transcription, from the control of cell survival to cross-talk with multiple other signalling pathways. In eukaryotic cells, cAMP activates three ubiquitously expressed intracellular cAMP effector proteins, the cAMP-dependent protein kinase A (PKA), the cyclic nucleotide gated ion channels, and the exchange protein activated by cAMP (Epac) . The presence of these three cAMP effector families contributes to convey a precise and integrated functional outcome to the cAMP signals. A fourth family of proteins, the Popeye domain containing proteins bind cAMP, although their specific function remains unclear.