Carnitine Palmitoyl Transferase-I (CPT-I) Activity Colorimetric Assay Kit (E-BC-K1107-M)
For research use only.
| Detection Principle |
Carnitine Palmitoyl Transferase-I (CPT-I) is a key rate-limiting enzyme in the fatty acid β-oxidation process. Its mechanism of action is closely related to its core function in fatty acid β-oxidation, involving substrate recognition, acyl transfer, and the synergistic effect of regulatory factors. CPT-I catalyzes the formation of acyl-carnitine from fatty acyl-CoA, and simultaneously reacts with a chromogenic agent to form TNB, which causes an increase in absorbance at 412 nm. The activity of CPT-I can be determined by measuring the rate of the increase in absorbance at 412 nm. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (412 nm) |
| Research Area | Metabolic Diseases,Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.60 U/L |
| Detection Range | 2.60-155.7 U/L |
| Precision | inter-assay CV: 6.5%-9.6% | intra-assay CV: 2.7%-3.8% |
| Sample Volume | 10 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1-2 |
| 10% Mouse kindey tissue homogenate | 1-2 |
| 1×10^7 A549 cells | 1 |
| 1×10^7 293T cells | 1 |
| 1×10^7 HL60 cells | 1 |
| 1×10^7 K562 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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