Caspase 8 Activity Assay Kit(Colorimetric Method) (E-CK-A388)
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For research use only.
| Detection Principle | Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm. |
| Detection Method | Colorimetric method |
| Sample Type | Cell and tissue samples |
| Assay Time | 4-6 hours |
| Detection Instrument | Spectrophotometer;Microplate reader |
| Other Reagents Required | PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
| Cat.No. | Product Name | Clone No. |
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