Cell Glutathione Peroxidase (GPX) Activity Assay Kit (E-BC-K809-M)
For research use only.
| Detection Principle | GPX catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents. The reducing agent has a maximum absorbance at 340 nm. The activity of GPX can be calculated by measuring the change of absorbance value at 340 nm. |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.22 U/L |
| Detection Range | 3.22-446.95 U/L |
| Precision | inter-assay CV: 0.4-3.4% | intra-assay CV: 1.2-3.1% |
| Sample Volume | 20 μL(cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1.9×10^6 HL-60 cells | 1 |
| 1.4×10^6 293T cells | 1 |
| 2×10^6个CHO cells | 1 |
| 1.9 × 10^6 4T1 cells | 1 |
| 2.03 × 10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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