Citrate Fluorometric Assay Kit (E-BC-F153)
For research use only.
| Detection Principle |
Citrate is widely present in animal blood, tissues, and plant fruits. In living organisms, citrate is typically synthesized from oxaloacetate and acetyl-CoA via citrate synthase. As an intermediate metabolite in the tricarboxylic acid (TCA) cycle, CA can be rapidly utilized to generate ATP under stress conditions, thereby enhancing the organism's resistance to stress. Citrate is converted into oxaloacetate under the catalysis of citrate lyase. Oxaloacetate is subsequently transformed into hydrogen peroxide (H2O2) via multiple enzymatic reactions. The generated H2O2 reacts with the fluorescent probe ADHP to produce a fluorescent substance. The fluorescence intensity can be measured to calculate the citrate content in the sample. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Other Reagents Required | Distilled or deionized water, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.81 μmol/L |
| Detection Range | 0.81-100 μmol/L |
| Precision | inter-assay CV: 1.1%-5.8% | intra-assay CV: 0.8%-1.3% |
| Sample Volume | 100-200 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse spleen tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Orange pulp tissue homogenization | 5-40 |
| 10% Orange peel tissue homogenization | 2-10 |
| Human serum | 2-20 |
| Rat serum | 2-5 |
| Pig serum | 1 |
| Horse serum | 1 |
| Human milk | 8-20 |
| 1×10^6 293T cells | 1 |
| 1×10^6 RAW 264.7 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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