Creatine Colorimetric Assay Kit(Enzyme Method) (E-BC-K900-M)
For research use only.
| Detection Principle |
Creatine is a nitrogen-containing organic acid that can assist in providing energy for muscle and nerve cells. Creatine is mainly stored in muscle tissue. It can relieve muscle fatigue and tension, enhance muscle elasticity, make muscles firmer, accelerate the synthesis of human proteins, lower cholesterol, blood lipids and blood sugar, and delay the aging of the body. Creatine is catalyzed by enzymes to produce hydrogen peroxide. Hydrogen peroxide reacts with a chromogenic agent to form a pink substance. The content of creatine can be calculated by measuring the change in OD value at a wavelength of 515 nm. |
| Sample Type | Animal tissue,Urine,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (730-770 nm, optimum wavelength: 750 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.04-1 mmol/L |
| Precision | inter-assay CV: 3.6%-7.3% | intra-assay CV: 0.3%-0.7% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1-2 |
| Rat serum | 1-2 |
| Human serum | 1 |
| Urine | 5-10 |
| 10% Mouse muscle tissue homogenate | 3-5 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® 430
Elab Fluor® 488
Elab Fluor® 568
Elab Fluor® 594
Elab Fluor® 647
Elab Fluor® 680
Elab Fluor® 700
Elab Fluor® 750
Elab Fluor® Red 780
Elab Fluor® Violet 450
Elab Fluor®488
Elab Fluor®594
Elab Fluor®647
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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