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Creatine Colorimetric Assay Kit(Enzyme Method) (E-BC-K900-M)

Creatine Colorimetric Assay Kit(Enzyme Method) - 1
  • Creatine Colorimetric Assay Kit(Enzyme Method) - 1
  • Creatine Colorimetric Assay Kit(Enzyme Method) - 2
  • Creatine Colorimetric Assay Kit(Enzyme Method) - 3
All Size Price Qty
96T $ 320.00
- +
48T $ 220.00
- +
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For research use only.

Detection Principle Creatine is a nitrogen-containing organic acid that can assist in providing energy for muscle and nerve cells. Creatine is mainly stored in muscle tissue. It can relieve muscle fatigue and tension, enhance muscle elasticity, make muscles firmer, accelerate the synthesis of human proteins, lower cholesterol, blood lipids and blood sugar, and delay the aging of the body.
Creatine is catalyzed by enzymes to produce hydrogen peroxide. Hydrogen peroxide reacts with a chromogenic agent to form a pink substance. The content of creatine can be calculated by measuring the change in OD value at a wavelength of 515 nm.
Sample Type Animal tissue,Urine,Serum,Plasma
Detection Method Colorimetric method
Detection Instrument Microplate reader (730-770 nm, optimum wavelength: 750 nm)
Research Area Amino Acids And Proteins
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.01 mmol/L
Detection Range 0.04-1 mmol/L
Precision inter-assay CV: 3.6%-7.3% | intra-assay CV: 0.3%-0.7%
Sample Volume 10 μL
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K558-M Creatine Kinase (CK) Activity Assay Kit 96T , 48T
E-BC-K558-S Creatine kinase (CK) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Mouse serum 1-2
Rat serum 1-2
Human serum 1
Urine 5-10
10% Mouse muscle tissue homogenate 3-5
10% Mouse kidney tissue homogenate 1
10% Mouse brain tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® 430

Elab Fluor® 488

Elab Fluor® 568

Elab Fluor® 594

Elab Fluor® 647

Elab Fluor® 680

Elab Fluor® 700

Elab Fluor® 750

Elab Fluor® Red 780

Elab Fluor® Violet 450

Elab Fluor®488

Elab Fluor®594

Elab Fluor®647

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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