Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method) (E-BC-K188-M)
For research use only.
| Detection Principle | Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm. |
| Synonyms | Cr |
| Sample Type | Serum,Plasma,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.8 μmol/L |
| Detection Range | 20.45-400 μmol/L |
| Precision | inter-assay CV: 3.7% | intra-assay CV: 1.4% |
| Sample Volume | 12 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human urine | 40-60 |
| Human serum | 1 |
| Rat serum | 1 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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