Diamine Oxidase (DAO) Activity Assay Kit (E-BC-K524-M)
For research use only.
| Detection Principle | Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time. |
| Synonyms | DAO |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450-470 nm, optimum wavelength: 460 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 7.39 U/L |
| Detection Range | 7.39-134.15 U/L |
| Precision | inter-assay CV: 0.07 | intra-assay CV: 0.05 |
| Sample Volume | 40 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-4 |
| Human plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| Human urine | 1 |
| Hela Cells (1 × 10^6) | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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