Ferroptosis Suppressor Protein-1 (FSP-1) Activity Fluorometric Assay Kit (E-BC-F022)
For research use only.
| Detection Principle |
Ferroptosis inhibitory protein-1(FSP-1) induce non-caspase-dependent apoptosis based on factors such as C-terminal fragment of amino acid series, nuclear translocation and overexpression. Through the FSP1-CoQ10-NAD(P)H pathway and the classical glutathione (GSH)-GPX4 pathway, FSP-1 can protect cells from ferroptosis, and plays a "double-edged sword" role in cell life activities. The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535 nm and emission wavelength of 587 nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value. |
| Sample Type | Animal and plant tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Ferroptosis |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.15 U/L |
| Detection Range | 0.15-5.00 U/L |
| Precision | inter-assay CV: 6.5-8.0% | intra-assay CV: 3.0-5.4% |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-5 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1-2 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Green beans tissue homogenate | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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