Fructokinase (FRK) Activity Colorimetric Assay Kit (E-BC-K1157-M)
For research use only.
| Detection Principle |
Fructokinase (FRK) is one of the key enzymes in the body's sugar metabolism, catalyzing the conversion of fructose into fructose-6-phosphate (F6P), which then proceeds to the glycolysis pathway and the oxidative pentose pathway. The detection principle of this kit: The product generated by FRK catalyzing the substrate has the maximum absorption at 340 nm. The FRK enzyme activity is calculated by measuring the OD value at 340 nm. |
| Sample Type | Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader(340 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.61 U/L |
| Detection Range | 1.61-83.76 U/L |
| Precision | inter-assay CV: 8.0%-9.6% | intra-assay CV: 1.5%-3.3% |
| Sample Volume | 20 μL(tissue homogenate/cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Yeast powder | 10-15 |
| 10% Mouse kidney tissue homogenate | 5-10 |
| 1×10^6 A549 cells | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 K562 cells | 1 |
| 10% Potato tissue homogenate | 1 |
| 10% Tomato pulp tissue homogenate | 1 |
| 10% Yellow peach pulp tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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