Glycogen Synthase(GCS) Activity Colorimetric Assay Kit (E-BC-K1154-M)
For research use only.
| Detection Principle |
Glycogen synthase (GCS) adds the sugar group of UDPG to the non-reducing end of the existing glycogen or glycogen protein, linking them through an α-1,4 glycosidic bond.GCS is the rate-limiting enzyme in the glycogen synthesis process of the animal body and also the main target enzyme for insulin action. It plays an important role in sugar metabolism and maintaining relatively stable blood sugar levels. GCS catalyzes substrate reactions to consume NADH.Measuring the decline rate of NADH at 340 nm can reflect the activity of GCS. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Other Reagents Required | Normal saline (0.9% NaCl)，Double distilled water, |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.27 U/L |
| Detection Range | 1.27-214.46 U/L |
| Precision | inter-assay CV: 5.8%-9.7% | intra-assay CV: 3.5%-4.3% |
| Sample Volume | 20 μL(tissue/cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 2-3 |
| 10% Mouse muscle tissue homogenization | 5-10 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 K562 cells | 1 |
| 1×10^6 A549 cells | 1 |
| 1×10^6 RAW264.7 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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