High-Density Lipoprotein Chlesterol (HDL-C) Fluorometric Assay Kit (E-BC-F035)
For research use only.
| Detection Principle |
High-density lipoprotein cholesterol (HDL-C), mainly synthesized in the liver, is an anti-atherosclerotic lipoprotein composed of apolipoprotein, phospholipid, cholesterol and fatty acids. The role of HDL-C is to transport phospholipids and cholesterol, which can transport cholesterol from extrahepatic tissues to the liver for metabolism, and be excreted from the body by bile acids. Plasma level of HDL-C are inversely associated with the risk of cardiovascular disease. HDL can take cholesterol from cell membranes, catalyze it into cholesterol esters by lecithin cholesterol acyltransferase, and then transfer the cholesterol ester to very low density lipoproteins and low density lipoproteins. The cholesterol content of high-density lipoprotein is relatively fixed, about 20-30% of the total cholesterol of the human body. After a series of reactions of HDL-C, the product can form a complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured, and the content of HDL-C in the sample can be calculated. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), Isopropyl alcohol (AR) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.15 μmol/L |
| Detection Range | 0.15-295 μmol/L |
| Precision | inter-assay CV: 7.1-8.7% | intra-assay CV: 2.1-2.4% |
| Sample Volume | 10 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 8-15 |
| Rat serum | 2-6 |
| Porcine serum | 4-10 |
| Mouse serum | 4-10 |
| Rabbit serum | 4-10 |
| Horse serum | 2-6 |
| 10% Mouse liver tissue homogenate | 4-8 |
| 10% Mouse lung tissue homogenate | 6-12 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 3T3 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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