Triglyceride（TG）Fluorometric Assay Kit (E-BC-F033)
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For research use only.
| Detection Principle | Triglyceride is converted by enzyme to produce hydrolytic products, which is catalyzed by enzymes to produce fluorescent substances. The content of triglyceride in samples can be calculate by measuring the fluorescence value. |
| Synonyms | TG |
| Sample Type | Serum,Plasma,Tissue,Cell sample |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.8 μmol/L |
| Detection Range | 0.8-30 μmol/L |
| Precision | inter-assay CV: 9.1% | intra-assay CV: 3.9% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Porcine heart tissue homogenate | 3-6 |
| 10% Porcine liver tissue homogenate | 8-10 |
| 1 × 10^6 CHO cells | 1-2 |
| 1 × 10^6 293T cells | 1-2 |
| Human serum | 100-120 |
| Mouse serum | 100-120 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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