Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit (E-CL-H0189)

Name: Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0189
Price: 180.0 USD
Availability: InStock

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Human 15-LO (Arachidonate 15-Lipoxygenase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human 15-LO in samples.No significant cross-reactivity or interference between Human 15-LO and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51683.000	56761.000	54222.000	54185
500	21138.000	24282.000	22710.000	22673
250	10698.000	9972.000	10335.000	10298
125	5025.000	5117.000	5071.000	5034
62.5	2555.000	2389.000	2472.000	2435
31.25	1326.000	1268.000	1297.000	1260
15.63	730.000	766.000	748.000	711
0	36.000	38.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	46.100	155.710	334.110	50.640	154.800	344.540
Standard deviation	4.730	14.170	20.280	5.970	16.320	35.900
CV（%）	10.260	9.100	6.070	11.790	10.540	10.420

Recovery

The recovery of Human 15-LO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-107	101
EDTA plasma （n=8）	86-98	92
Cell culture media （n=8）	99-110	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human 15-LO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	85-100	86-98
1:2	Average	91	91	93
1:4	Range	90-104	83-99	87-102
1:4	Average	97	90	94
1:8	Range	85-101	96-114	96-110
1:8	Average	92	104	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 15-LO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 15-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 15-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 15-LO. You can calculate the concentration of Human 15-LO in the samples by comparing the RLU value of the samples to the standard curve.