Human 26S-PSM (26S Proteasome) CLIA Kit (E-CL-H0193)

Name: Human 26S-PSM (26S Proteasome) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0193
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human 26S-PSM (26S Proteasome) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human 26S-PSM in samples.No significant cross-reactivity or interference between Human 26S-PSM and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	60488.000	66324.000	63406.000	63369
2000	27945.000	31773.000	29859.000	29822
1000	14024.000	13710.000	13867.000	13830
500	6325.000	7183.000	6754.000	6717
250	3714.000	3204.000	3459.000	3422
125	1731.000	1649.000	1690.000	1653
62.5	733.000	805.000	769.000	732
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	220.870	606.070	1409.500	221.680	660.500	1495.520
Standard deviation	26.460	71.270	122.200	18.730	74.310	96.310
CV（%）	11.980	11.760	8.670	8.450	11.250	6.440

Recovery

The recovery of Human 26S-PSM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-108	102
EDTA plasma （n=8）	88-100	93
Cell culture media （n=8）	88-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human 26S-PSM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	85-95	90-102
1:2	Average	103	90	96
1:4	Range	88-101	88-103	100-117
1:4	Average	95	94	108
1:8	Range	88-99	90-107	102-114
1:8	Average	94	97	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 26S-PSM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 26S-PSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 26S-PSM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 26S-PSM. You can calculate the concentration of Human 26S-PSM in the samples by comparing the RLU value of the samples to the standard curve.