Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit (E-CL-H0423)

Name: Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0423
Price: 180.0 USD
Availability: InStock

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Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ABCG1 in samples.No significant cross-reactivity or interference between Human ABCG1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	51688.000	61580.000	56634.000	56605
1000	23666.000	27804.000	25735.000	25706
500	11610.000	11170.000	11390.000	11361
250	5336.000	6026.000	5681.000	5652
125	3000.000	2628.000	2814.000	2785
62.5	1442.000	1244.000	1343.000	1314
31.25	690.000	758.000	724.000	695
0	29.000	29.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.360	258.810	673.220	101.870	238.810	618.160
Standard deviation	8.560	25.910	50.760	12.470	27.100	43.460
CV（%）	8.700	10.010	7.540	12.240	11.350	7.030

Recovery

The recovery of Human ABCG1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-101	94
EDTA plasma （n=8）	87-101	93
Cell culture media （n=8）	85-98	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ABCG1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-105	93-108	89-104
1:2	Average	96	99	96
1:4	Range	96-114	98-113	91-106
1:4	Average	104	105	97
1:8	Range	86-101	89-100	98-114
1:8	Average	93	94	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ABCG1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ABCG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ABCG1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ABCG1. You can calculate the concentration of Human ABCG1 in the samples by comparing the RLU value of the samples to the standard curve.

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