Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit (E-CL-H1143)

Name: Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1143
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACP3/PAP in samples.No significant cross-reactivity or interference between Human ACP3/PAP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	31341.000	33787.000	32564.000	32537
5	16036.000	17244.000	16640.000	16613
2.5	8613.000	7807.000	8210.000	8183
1.25	3719.000	4291.000	4005.000	3978
0.63	1852.000	1780.000	1816.000	1789
0.31	875.000	787.000	831.000	804
0.16	359.000	371.000	365.000	338
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.470	1.320	3.480	0.510	1.340	3.390
Standard deviation	0.060	0.130	0.320	0.060	0.140	0.220
CV（%）	12.770	9.850	9.200	11.760	10.450	6.490

Recovery

The recovery of Human ACP3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-109	102
EDTA plasma （n=8）	92-104	99
Cell culture media （n=8）	90-105	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACP3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-109	87-100	91-106
1:2	Average	101	93	98
1:4	Range	86-99	86-98	96-110
1:4	Average	93	92	103
1:8	Range	100-113	93-109	95-105
1:8	Average	106	101	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACP3/PAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACP3/PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACP3/PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACP3/PAP. You can calculate the concentration of Human ACP3/PAP in the samples by comparing the RLU value of the samples to the standard curve.

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