Human ACTG1 (Actin Gamma 1) CLIA Kit (E-CL-H0213)

Name: Human ACTG1 (Actin Gamma 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0213
Price: 180.0 USD
Availability: InStock

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Human ACTG1 (Actin Gamma 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACTG1 in samples.No significant cross-reactivity or interference between Human ACTG1 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	53266.000	63370.000	58318.000	58287
500	22028.000	23096.000	22562.000	22531
250	11285.000	9479.000	10382.000	10351
125	5065.000	5543.000	5304.000	5273
62.5	2793.000	2549.000	2671.000	2640
31.25	1482.000	1298.000	1390.000	1359
15.63	710.000	774.000	742.000	711
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.870	133.440	374.960	49.330	134.020	386.930
Standard deviation	4.940	15.680	42.030	5.340	11.240	31.650
CV（%）	9.710	11.750	11.210	10.830	8.390	8.180

Recovery

The recovery of Human ACTG1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	96
EDTA plasma （n=8）	95-110	103
Cell culture media （n=8）	89-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACTG1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-107	101-119	93-110
1:2	Average	98	108	100
1:4	Range	94-108	89-99	90-102
1:4	Average	101	94	96
1:8	Range	100-113	94-109	90-104
1:8	Average	106	100	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTG1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTG1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACTG1. You can calculate the concentration of Human ACTG1 in the samples by comparing the RLU value of the samples to the standard curve.

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