Human β-actin(Actin Beta) CLIA Kit (E-CL-H0672)

Name: Human β-actin(Actin Beta) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0672
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human β-actin in samples.No significant cross-reactivity or interference between Human β-actin and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	34292.000	34764.000	34528.000	34467
1000	16060.000	16930.000	16495.000	16434
500	7630.000	7920.000	7775.000	7714
250	3737.000	3615.000	3676.000	3615
125	1961.000	1877.000	1919.000	1858
62.5	873.000	833.000	853.000	792
31.25	330.000	370.000	350.000	289
0	64.000	58.000	61.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	88.970	201.870	824.650	86.240	218.680	887.420
Standard deviation	5.100	9.570	43.460	4.310	10.890	36.030
CV（%）	5.730	4.740	5.270	5.000	4.980	4.060

Recovery

The recovery of Human β-actin was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-97	94
EDTA plasma （n=8）	89-100	97
Cell culture media （n=8）	95-104	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human β-actin and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-108	98-109	88-101
1:2	Average	101	102	93
1:4	Range	99-111	87-98	99-111
1:4	Average	105	92	105
1:8	Range	84-99	89-100	86-96
1:8	Average	91	96	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human β-actin. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human β-actin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human β-actin, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human β-actin. You can calculate the concentration of Human β-actin in the samples by comparing the RLU value of the samples to the standard curve.