Human ACTN2 (Actinin Alpha 2) CLIA Kit (E-CL-H0214)

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Size	Price	Qty
96T	$ 682.00
48T	$ 546.00
24T	$ 180.00
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes Human ACTN2 in samples.No significant cross-reactivity or interference between Human ACTN2 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACTN2. You can calculate the concentration of Human ACTN2 in the samples by comparing the RLU value of the samples to the standard curve.

Background

Uniport ID	P35609
Research Area	Cancer , Cardiovascular , Signal Transduction

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Human ACTN2 (Actinin Alpha 2) CLIA Kit (E-CL-H0214)

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