Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit (E-CL-H0222)

Name: Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0222
Price: 180.0 USD
Availability: InStock

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Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADAMTS4 in samples.No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	56724.000	57876.000	57300.000	57275
500	21418.000	24898.000	23158.000	23133
250	10914.000	10520.000	10717.000	10692
125	4808.000	5662.000	5235.000	5210
62.5	2738.000	2436.000	2587.000	2562
31.25	1388.000	1228.000	1308.000	1283
15.63	732.000	792.000	762.000	737
0	25.000	25.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.560	133.210	498.450	44.760	134.870	472.870
Standard deviation	4.960	10.140	51.640	4.390	15.470	52.490
CV（%）	10.430	7.610	10.360	9.810	11.470	11.100

Recovery

The recovery of Human ADAMTS4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-114	105
EDTA plasma （n=8）	87-103	94
Cell culture media （n=8）	90-104	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADAMTS4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-101	90-106	91-108
1:2	Average	95	97	99
1:4	Range	90-101	99-114	88-103
1:4	Average	96	106	94
1:8	Range	90-105	98-113	101-115
1:8	Average	96	106	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADAMTS4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAMTS4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAMTS4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADAMTS4. You can calculate the concentration of Human ADAMTS4 in the samples by comparing the RLU value of the samples to the standard curve.

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