Human ADH1 (Alcohol Dehydrogenase 1) CLIA Kit (E-CL-H0224)

Name: Human ADH1 (Alcohol Dehydrogenase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0224
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ADH1 (Alcohol Dehydrogenase 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADH1 in samples.No significant cross-reactivity or interference between Human ADH1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53622.000	64368.000	58995.000	58959
1000	23835.000	24781.000	24308.000	24272
500	12182.000	10210.000	11196.000	11160
250	5394.000	6054.000	5724.000	5688
125	3017.000	2579.000	2798.000	2762
62.5	1346.000	1294.000	1320.000	1284
31.25	721.000	751.000	736.000	700
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	110.360	168.840	853.180	112.710	173.880	870.220
Standard deviation	13.640	13.710	78.320	14.440	17.940	64.140
CV（%）	12.360	8.120	9.180	12.810	10.320	7.370

Recovery

The recovery of Human ADH1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	104
EDTA plasma （n=8）	97-112	104
Cell culture media （n=8）	86-96	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADH1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	83-95	94-107
1:2	Average	95	90	100
1:4	Range	97-114	88-98	98-116
1:4	Average	104	93	106
1:8	Range	87-99	95-105	91-104
1:8	Average	92	100	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADH1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADH1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADH1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADH1. You can calculate the concentration of Human ADH1 in the samples by comparing the RLU value of the samples to the standard curve.